<rss xmlns:atom="http://www.w3.org/2005/Atom" version="2.0"><channel><title>MiXCR - Tag - Xiaopeng Xu</title><link>https://xu-xp.com/tags/mixcr/</link><description>MiXCR - Tag - Xiaopeng Xu</description><generator>Hugo -- gohugo.io</generator><language>en</language><managingEditor>xiaopeng.xu@kaust.edu.sa (Xiaopeng Xu)</managingEditor><webMaster>xiaopeng.xu@kaust.edu.sa (Xiaopeng Xu)</webMaster><lastBuildDate>Thu, 08 Feb 2024 00:00:00 +0000</lastBuildDate><atom:link href="https://xu-xp.com/tags/mixcr/" rel="self" type="application/rss+xml"/><item><title>Ig-seq 操作说明</title><link>https://xu-xp.com/posts/ig-seq-mixcr/</link><pubDate>Thu, 08 Feb 2024 00:00:00 +0000</pubDate><author>xiaopeng.xu@kaust.edu.sa (Xiaopeng Xu)</author><guid>https://xu-xp.com/posts/ig-seq-mixcr/</guid><description><![CDATA[<h2 id="数据下载">数据下载</h2>
<p>从 NCBI SRA 平台下载对应的 Ig seq 项目数据。</p>
<ul>
<li><a href="https://www.ncbi.nlm.nih.gov/Traces/study/" target="_blank" rel="noopener noreffer ">https://www.ncbi.nlm.nih.gov/Traces/study/</a></li>
</ul>
<p>先导入 google cloud 的 bucket 中，然后通过 gsutils 下载到本地或 ibex 文件夹中。</p>
<h2 id="mixcr-数据分析">MiXCR 数据分析</h2>
<h3 id="安装--mixcr">安装  MiXCR</h3>
<div class="code-block code-line-numbers open" style="counter-reset: code-block 0">
    <div class="code-header language-powershell">
        <span class="code-title"><i class="arrow fas fa-angle-right" aria-hidden="true"></i></span>
        <span class="ellipses"><i class="fas fa-ellipsis-h" aria-hidden="true"></i></span>
        <span class="copy" title="Copy to clipboard"><i class="far fa-copy" aria-hidden="true"></i></span>
    </div><div class="highlight"><pre tabindex="0" class="chroma"><code class="language-powershell" data-lang="powershell"><span class="line"><span class="cl"><span class="n">conda</span> <span class="n">install</span> <span class="n">-c</span> <span class="nb">imperial-college</span><span class="n">-research-computing</span> <span class="n">mixcr</span></span></span></code></pre></div></div>
<h3 id="ig-seq-快速使用">Ig-seq 快速使用</h3>
<div class="code-block code-line-numbers open" style="counter-reset: code-block 0">
    <div class="code-header language-powershell">
        <span class="code-title"><i class="arrow fas fa-angle-right" aria-hidden="true"></i></span>
        <span class="ellipses"><i class="fas fa-ellipsis-h" aria-hidden="true"></i></span>
        <span class="copy" title="Copy to clipboard"><i class="far fa-copy" aria-hidden="true"></i></span>
    </div><div class="highlight"><pre tabindex="0" class="chroma"><code class="language-powershell" data-lang="powershell"><span class="line"><span class="cl"><span class="c"># Align</span>
</span></span><span class="line"><span class="cl"><span class="n">Align</span><span class="err">:</span> <span class="n">mixcr</span> <span class="n">align</span><span class="err">–</span><span class="n">library</span> <span class="n">my_library</span> <span class="n">-t</span> <span class="mf">8</span> <span class="n">-r</span> <span class="n">align_log</span><span class="p">.</span><span class="py">txt</span> <span class="n">R1</span> <span class="n">R2</span> <span class="n">alignments</span><span class="p">.</span><span class="py">vdjca</span> <span class="n">-s</span> <span class="n">hs</span>
</span></span><span class="line"><span class="cl"><span class="c"># What is the my_library used here</span>
</span></span><span class="line"><span class="cl">
</span></span><span class="line"><span class="cl"><span class="c"># Assemble</span>
</span></span><span class="line"><span class="cl"><span class="n">Assemble</span><span class="err">:</span> <span class="n">mixcr</span> <span class="n">assemble</span> <span class="n">-r</span> <span class="n">assemble_log</span><span class="p">.</span><span class="py">txt</span> <span class="n">-OseparateByV</span> <span class="p">=</span> <span class="n">true</span> <span class="n">-OseparateByJ</span> <span class="p">=</span> <span class="n">true</span> <span class="n">-OseparateByC</span> <span class="p">=</span> <span class="n">true</span> <span class="n">align</span><span class="p">-</span>
</span></span><span class="line"><span class="cl"><span class="n">ments</span><span class="p">.</span><span class="py">vdjca</span> <span class="n">clones</span><span class="p">.</span><span class="n">clns</span></span></span></code></pre></div></div>
<h3 id="使用介绍">使用介绍</h3>
<p>典型的 MiXCR 工作流程主要由三个部分构成：</p>]]></description></item></channel></rss>